ERp57 Polyclonal Antibody

Western Blot analysis of A549, HT29, K562 cells using ERp57 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	P30101
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	56782 Da

Additional Information
Gene ID	2923
Other Names	PDIA3; ERP57; ERP60; GRP58; Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60;Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
Dilution	WB~~Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. IHC-P~~Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	PDIA3 (HGNC:4606)
Synonyms	ERP57, ERP60, GRP58
Function	Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed:11825568, PubMed:16193070, PubMed:27897272, PubMed:36104323, PubMed:7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed:35948544, PubMed:36104323).
Cellular Location	Endoplasmic reticulum. Endoplasmic reticulum lumen {ECO:0000250\|UniProtKB:P11598}. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Tissue Location	Detected in the flagellum and head region of spermatozoa (at protein level) (PubMed:20400973). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level) (PubMed:24188822)