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MPO Polyclonal Antibody

     
  • 1 - MPO Polyclonal Antibody AP73534
    Western Blot analysis of K562 cells using MPO Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
  • 14 - MPO Polyclonal Antibody AP73534
    Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - MPO Polyclonal Antibody AP73534
    Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - MPO Polyclonal Antibody AP73534
    Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - MPO Polyclonal Antibody AP73534
    Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - MPO Polyclonal Antibody AP73534
    Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - MPO Polyclonal Antibody AP73534
    Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P
Primary Accession P05164
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 83869 Da
Additional Information
Gene ID 4353
Other Names MPO; Myeloperoxidase; MPO
Dilution WB~~IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
IHC-P~~IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name MPO (HGNC:7218)
Function Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Cellular Location Lysosome.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

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