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APOE Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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All lanes : Anti-APOE Antibody (C-term) at 1:2000 dilution Lane 1: Human plasma whole lysate Lane 2: Mouse plasma whole lysate Lane 3: Mouse liver whole lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-APOE Antibody (C-term) at 1:1000 dilution Lane 1: Human plasma lysate Lane 2: Mouse plasma lysate Lane 3: Human ovary lysate Lane 4: Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysate from human plasma tissue lysate, using APOE Antibody (C-term)(Cat. #AP7587b). AP7587b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot analysis of lysate from human blood plasma tissue lysate, using APOE Antibody (C-term)(Cat. #AP7587b). AP7587b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Western blot analysis of APOE(arrow) using rabbit polyclonal APOE Antibody (C-term) (Cat.#AP7587b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APOE gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOE antibody (C-term) (Cat.#AP7587b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P02649
Other Accession	P18287, P10517
Reactivity	Human, Rat, Mouse
Predicted	Monkey, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	263-292 aa

Additional Information
Other Names	Apolipoprotein E, Apo-E, APOE
Target/Specificity	This APOE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human APOE.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	APOE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III),in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

REFERENCES

Wood,K.C., Appl Physiol Nutr Metab 33 (4), 761-768 (2008) Pemberton,L., (er) HIV Med. (2008) In press Sundermann,E.E., (er) Horm Behav (2008) In press Christensen,H.,(er) BMC Geriatr 8, 14 (2008)

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Species tested

Human

Application tested

Organization tested

U87,LO2,A549,293T

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Brief protocol

1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose

¥ 1,250.00

Cat# AP7587b

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

询价

APOE Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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