RPS6 Rabbit mAb

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using RPS6 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Western blot analysis of S6 Ribosomal Protein in Ramos, Jurkat, C6, 3T3 and Hela lysates using S6 Ribosomal Protein antibody.
Immunocytochemistry analysis of HeLa using antiS6 Ribosomal Protein antibody.
Immunoprecipitation analysis of Hela cell lysates using S6 Ribosomal Protein antibody.
Western blot analysis of S6 Ribosomal Protein in CHO-K1, Raji, Raw264.7 and COS7 lysates using S6 Ribosomal Protein anibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC
Primary Accession	P62753
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	28681 Da

Additional Information
Gene ID	6194
Other Names	RPS6
Dilution	WB~~1:500-1:1000 IHC-P~~1:50~200 FC~~1:200-1:500
Format	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	RPS6 {ECO:0000303\|PubMed:29563586, ECO:0000312\|HGNC:HGNC:10429}
Function	Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Cellular Location	Cytoplasm. Nucleus, nucleolus

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.