EPHA10 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysates from Hela, 293T cell line (from left to right), using EPHA10 Antibody (N-term)(Cat. #AP7614a). AP7614a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot analysis of EPHA10 Antibody (N-term) (Cat.#AP7614a) in K562 cell line lysates (35ug/lane). EPHA10 (arrow) was detected using the purified Pab.
Western blot analysis of EPHA10 (arrow) using rabbit polyclonal EPHA10 Antibody (N-term) (Cat. #AP7614a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml)
Formalin-fixed and paraffin-embedded human testis tissue reacted with EPHA10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q5JZY3
Other Accession	Q8BYG9, P54759, Q61772, Q15375, O42422
Reactivity	Human
Predicted	Chicken, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	109716 Da
Antigen Region	149-179 aa

Additional Information
Gene ID	284656
Other Names	Ephrin type-A receptor 10, EPHA10
Target/Specificity	This EPHA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-179 amino acids from the N-terminal region of human EPHA10.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	EPHA10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	EPHA10
Function	Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5.
Cellular Location	[Isoform 1]: Cell membrane; Single- pass type I membrane protein [Isoform 2]: Secreted.
Tissue Location	Mainly expressed in testis.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.