Sestrin-1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of Sestrin-1 antibody (C-term) (Cat.#AP7650b) in mouse heart tissue lysates (35ug/lane). Sestrin-1 (arrow) was detected using the purified Pab.
Sestrin-1 Antibody (C-term) (Cat.#AP7650b) western blot analysis in NCI-H460,HL-60 cell line lysates (35ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).
Sestrin-1 Antibody (C-term) (Cat. #AP7650b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q9Y6P5
Other Accession	P58003, P58006, Q4R6P7
Reactivity	Human, Mouse
Predicted	Monkey, Xenopus
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	56557 Da
Antigen Region	312-341 aa

Additional Information
Gene ID	27244
Other Names	Sestrin-1, p53-regulated protein PA26, SESN1, PA26, SEST1
Target/Specificity	This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-341 amino acids from the C-terminal region of human Sestrin-1.
Dilution	WB~~1:1000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Sestrin-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SESN1 (HGNC:21595)
Function	Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:25263562, PubMed:26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (By similarity).
Cellular Location	Nucleus. Cytoplasm
Tissue Location	Widely expressed..