癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
HLF Rabbit mAb
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Application 
| WB |
|---|---|
| Primary Accession | Q16534 |
| Reactivity | Rat, Human, Mouse |
| Host | Rabbit |
| Clonality | Monoclonal Antibody |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Affinity Purified |
| Calculated MW | 33199 Da |
| Gene ID | 3131 |
|---|---|
| Other Names | HLF |
| Dilution | WB~~1:1000 |
| Format | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | HLF {ECO:0000303|PubMed:1516826, ECO:0000312|HGNC:HGNC:4977} |
|---|---|
| Function | Transcription factor which selectively binds with high affinity to the consensus sequence 5'-GTTACGTAAT-3' and to closely related sites (PubMed:8065331, PubMed:8164688, PubMed:8639829). Has transcriptional activator activity (PubMed:10073576, PubMed:8065331, PubMed:8164688, PubMed:8639829). Also has transcriptional repressor activity (By similarity). Binds to multiple sites in the promoter regions of coagulation factors F8 and F9 and activates transcription; transactivation activity is enhanced by formation of a heterodimer with DBP (PubMed:10073576). Binds to the promoter region of IRF2 and activates transcription (By similarity). Maintains quiescence of hematopoietic stem cells under steady-state conditions and protects the stem cell pool from toxic insults (By similarity). Regulates the tissue residency and proinflammatory function of CD4+ tissue-resident memory T (TRM) cells (By similarity). Down-regulates the expression of genes involved in promoting tissue egress of CD4+ TRM cells including S1PR1, KLF2 and TCF7 while up-regulating expression of tissue retention molecules such as CD69 (By similarity). Also up-regulates BHLHE40 expression, promoting proinflammatory cytokine production (By similarity). |
| Cellular Location | Nucleus. |
| Tissue Location | Highly expressed in liver; lower levels in lung and kidney |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Hepatic leukemia factor (Hlf), is a novel hepatic protein of the proline and acidic amino acid-rich basic leucine zipper (bZip) family, previously studied for its role in circadian rhythm regulated neurotransmitter metabolism in the brain (PMID: 29166614). HLF is an essential regulator of HSC quiescence and that HLF deficiency leads to an exhaustion of HSCs in adult mouse BM during regeneration or following cytotoxic stress (PMID: 29262330). Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors (PMID: 1516826). Western blot analysis detected bands 33 kDa whereas the 66 kDa could be the dimer form of Hlf.
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