GSDMD Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P57764
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal Antibody
Calculated MW	52801 Da

Protein Information
Name	GSDMD {ECO:0000303\|PubMed:26375003, ECO:0000312\|HGNC:HGNC:25697}
Function	[Gasdermin-D]: Precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27281216). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:26375259, PubMed:27281216).
Cellular Location	[Gasdermin-D]: Cytoplasm, cytosol. Inflammasome {ECO:0000250\|UniProtKB:Q9D8T2}. Note=In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. {ECO:0000250\|UniProtKB:Q9D8T2} [Gasdermin-D, N-terminal]: Cytoplasm, cytosol. Note=(Microbial infection) Upon infection by M.tuberculosis, localization to cell membrane is prevented by M.tuberculosis phosphatase PtpB that catalyzes dephosphorylation of phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol 4- phosphate, thereby inhibiting the membrane targeting of Gasdermin-D, N- terminal and subsequent cytokine release and pyroptosis [Gasdermin-D, C-terminal]: Cytoplasm, cytosol {ECO:0000250\|UniProtKB:Q9D8T2}
Tissue Location	Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells