ZFP36 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

Anti-ZFP36 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ZFP36 Antibody (Center) (Cat. #AP8520c) in mouse stomach tissue lysates (35ug/lane).ZFP36 (arrow) was detected using the purified Pab.(8ug/ml)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ZFP36 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ZFP36 Antibody (Center) (Cat.#AP8520c) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P16415
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	70270 Da
Antigen Region	119-148 aa

Additional Information
Gene ID	55552
Other Names	Zinc finger protein 823, Zinc finger protein ZFP-36, ZNF823, ZFP36
Target/Specificity	This ZFP36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human ZFP36.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ZFP36 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.