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FAS Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - FAS Antibody (Center) AP8530c
    Western blot analysis of FAS Antibody (Center) (Cat. #AP8530c) in T47D cell line lysates (35ug/lane).FAS (arrow) was detected using the purified Pab.
  • 1 - FAS Antibody (Center) AP8530c
    Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center) (Cat. #AP8530c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.
  • 3 - FAS Antibody (Center) AP8530c
    Confocal immunofluorescent analysis of FAS Antibody (Center)(Cat#AP8530c) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession P25445
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37732 Da
Additional Information
Gene ID 355
Other Names Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, TNFRSF6
Target/Specificity This FAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the Central region of human FAS.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FAS
Synonyms APT1, FAS1, TNFRSF6
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen- stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Membrane raft [Isoform 3]: Secreted. [Isoform 5]: Secreted.
Tissue Location Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Research Areas

BACKGROUND

FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.

REFERENCES

Feig,C., et.al., EMBO J. 26 (1), 221-231 (2007)
Jenkins,M., et.al., J. Biol. Chem. 275 (11), 7988-7993 (2000)

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