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USF2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - USF2 Antibody (Center) AP8585c
    Western blot analysis of USF2 Antibody (Center) (Cat. #AP8585c) in mouse cerebellum tissue lysates (35ug/lane).USF2 (arrow) was detected using the purified Pab.
  • 14 - USF2 Antibody (Center) AP8585c
    USF2 Antibody (Center) (Cat. #AP8585c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the USF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q15853
Other Accession Q63665, Q64705
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 36955 Da
Antigen Region 218-246 aa
Additional Information
Gene ID 7392
Other Names Upstream stimulatory factor 2, Class B basic helix-loop-helix protein 12, bHLHb12, FOS-interacting protein, FIP, Major late transcription factor 2, Upstream transcription factor 2, USF2, BHLHB12
Target/Specificity This USF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human USF2.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USF2
Synonyms BHLHB12
Function Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

USF2 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. This protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs.

REFERENCES

Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Groenen,P.M., et.al., Genomics 38 (2), 141-148 (1996)

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