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MPZ Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-MPZ Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: U-87MG whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: CCRF-CEM whole cell lysate Lane 6: Human brain whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MPZ Antibody (C-term) (Cat. #AP8621b) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MPZ Antibody (C-term) (Cat. #AP8621b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MPZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P, E
Primary Accession	P25189
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	27555 Da
Antigen Region	186-215 aa

Additional Information
Gene ID	4359
Other Names	Myelin protein P0, Myelin peripheral protein, MPP, Myelin protein zero, MPZ
Target/Specificity	This MPZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the C-terminal region of human MPZ.
Dilution	WB~~1:2000 FC~~1:10~50 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MPZ Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MPZ
Function	Is an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Found only in peripheral nervous system Schwann cells

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

MPZ is a major structural protein of peripheral myelin. Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.

REFERENCES

Tachi,N., et.al., Brain Dev. 6 (6), 560-565 (1984)
Hayasaka,K.,et.al., Biochem. Biophys. Res. Commun. 180 (2), 515-518 (1991)
Hayasaka,K., et.al., Genomics 17 (3), 755-758 (1993)

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¥ 1,250.00

Cat# AP8621b

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MPZ Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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