PTAR1 Antibody (Center) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-PTAR1 Antibody (Center) at 1:1000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of PTAR1 Antibody (Center) (Cat. #AP8622c) in HL-60 cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.
Western blot analysis of PTAR1 Antibody (Center) (Cat. #AP8622c) in Jurkat cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.
Western blot analysis of PTAR1 Antibody (Center) (Cat. #AP8622c) in MDA-MB231 cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma with PTAR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of SK-Br-3 cells using PTAR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q7Z6K3
Reactivity	Human
Predicted	Mouse, Rat, Bovine, Canine, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	46405 Da
Antigen Region	60-89 aa

Additional Information
Gene ID	375743
Other Names	Protein prenyltransferase alpha subunit repeat-containing protein 1, PTAR1
Target/Specificity	This PTAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the Central region of human PTAR1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PTAR1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PTAR1
Function	Substrate-recognition subunit of the geranylgeranyl transferase type 3 (GGTase-3) complex (PubMed:31209342, PubMed:32128853). The GGTase-3 complex geranylgeranylates and targets FBXL2 to the cellular membranes, where FBXL2 forms part of the E3 ubiquitin-protein ligase complex SCF(FBXL2) that mediates the degradation of membrane-anchored proteins (PubMed:31209342, PubMed:32128853). The GGTase-3 complex geranylgeranylates Golgi v-SNARE protein YKT6 at 'Cys-194' and this prenylation is required for Golgi SNARE complex assembly (PubMed:32128853).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.