BAT4 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of BAT4 Antibody (C-term) (Cat. #AP8721b) in mouse spleen tissue lysates (35ug/lane). BAT4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human spleen tissue reacted with BAT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BAT4 Antibody (C-term) (Cat. #AP8721b) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of BAT4 (arrow) using rabbit polyclonal BAT4 Antibody (C-term) (Cat. #AP8721b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BAT4 gene.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	O95872
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	39314 Da
Antigen Region	275-302 aa

Additional Information
Gene ID	7918
Other Names	G patch domain and ankyrin repeat-containing protein 1, Ankyrin repeat domain-containing protein 59, G patch domain-containing protein 10, HLA-B-associated transcript 4, Protein G5, GPANK1, ANKRD59, BAT4, G5, GPATCH10
Target/Specificity	This BAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-302 amino acids from the C-terminal region of human BAT4.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BAT4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.