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>   首页   >   产品   >   一抗   >   癌症   >   SOD1 Antibody (Center)   

SOD1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SOD1 Antibody (Center) AP8733c
    Western blot analysis of SOD1 Antibody (Center) (Cat. #AP8733c) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab.
  • 14 - SOD1 Antibody (Center) AP8733c
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - SOD1 Antibody (Center) AP8733c
    SOD1 Antibody (Center) (Cat. #AP8733c) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - SOD1 Antibody (Center) AP8733c
    Confocal immunofluorescent analysis of SOD1 Antibody (Center) (Cat. #AP8733c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, IF, E
Primary Accession P00441
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 15936 Da
Antigen Region 55-84 aa
Additional Information
Gene ID 6647
Other Names Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1
Target/Specificity This SOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human SOD1.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
IF~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOD1 (HGNC:11179)
Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein.

REFERENCES

Crapo,J.D., et.al., Proc. Natl. Acad. Sci. U.S.A. 89 (21), 10405-10409 (1992)

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