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>   首页   >   产品   >   重组兔单克隆抗体   >   重组兔单克隆抗体   >   B Raf Antibody   

B Raf Antibody

Rabbit mAb

     
  • 0 - B Raf Antibody AP90359
    Western blot analysis of B Raf expression in (1)Human brain lysate (2)HepG2 cell lysate.
  • 0 - B Raf Antibody AP90359
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, IP
Primary Accession P15056
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Other Names B-RAF; p94; NS7; B-Raf proto-oncogene serine/threonine-protein kinase;BRAF;BRAF1;
Isotype Rabbit IgG
Host Rabbit
Calculated MW 84437 Da
Additional Information
Dilution WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50 FC 1:50
Purification Affinity-chromatography
Immunogen A synthesized peptide derived from human B Raf
Description Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.
Storage Condition and Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Protein Information
Name BRAF (HGNC:1097)
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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