Firefly Luciferase Antibody
Rabbit mAb
- 产品详情
- 实验流程
Application ![]()
| WB, IF, ICC |
---|---|
Primary Accession | P08659 |
Clonality | Monoclonal |
Other Names | Luciferase; Firefly; Luciferin 4 monooxygenase; |
Isotype | Rabbit IgG |
Host | Rabbit |
Calculated MW | 60745 Da |
Dilution | WB 1:1000~1:5000 ICC/IF 1:50~1:200 |
---|---|
Purification | Affinity-chromatography |
Immunogen | A synthesized peptide derived from Firefly Luciferase |
Description | Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Name | LUCI |
---|---|
Function | Produces green light with a wavelength of 562 nm. |
Cellular Location | Peroxisome. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.

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Cat# AP90434