CD10 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC
Primary Accession	P08473
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	Atriopeptidase; CALLA; CD10; CD10 antigen;Enkephalinase; EPN; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); CALLA CD10; NEP; Neprilysin; Neutral endopeptidase;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	85514 Da

Additional Information
Dilution	WB 1:1000~1:2000 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CD10
Description	CD10 is a transmembrane type II molecule and functions as a metallo-peptidase requiring zinc. Specifically, CD10 cleaves 1-3 amino-terminal amino acids from peptides with a preference for neutral amino acids (valine, iso-leucine, phenylalanine, leucine or alanine). Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	MME {ECO:0000303\|PubMed:27588448, ECO:0000312\|HGNC:HGNC:7154}
Function	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
Cellular Location	Cell membrane; Single-pass type II membrane protein