CYP2E1 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P05181
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	Cytochrome P450 2E1; CYPIIE1; Cytochrome P450-J; CYP2E; P450C2E; CYPIIE1;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	56849 Da

Additional Information
Dilution	WB 1:500~1:2000
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CYP2E1
Description	Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CYP2E1 {ECO:0000303\|PubMed:10553002, ECO:0000312\|HGNC:HGNC:2631}
Function	A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10553002, PubMed:18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Microsome membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Mitochondrion inner membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Note=Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein {ECO:0000250\|UniProtKB:P05182}