癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Gli3 Antibody
Rabbit mAb
- 产品详情
- 实验流程
Application 
| WB, IF, ICC |
|---|---|
| Primary Accession | P10071 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Other Names | ACLS; GCPS; GLI3; GLI3FL; PAPA; PAPA1; PAPB; PHS; PPDIV; |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Calculated MW | 169863 Da |
| Dilution | WB 1:500~1:2000 ICC/IF 1:50~1:200 |
|---|---|
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human Gli3 |
| Description | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. |
| Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GLI3 {ECO:0000303|PubMed:2118997, ECO:0000312|HGNC:HGNC:4319} |
|---|---|
| Function | Transcription factor that acts as a key effector of the smoothened signaling pathway, and which plays a role in the development and patterning of various body structures, including the brain and limbs (PubMed:10075717, PubMed:10375510, PubMed:10693759, PubMed:11238441, PubMed:17764085, PubMed:20154143, PubMed:27146893). Specifically binds to the minimal GLI-consensus sequence 5'-GACCACCCA- 3' to activate or repress the expression of target genes (PubMed:10075717). Functions both as a transcriptional activator and repressor: the full-length GLI3 form (Transcription activator GLI3) acts as a transcription activator following smoothened activation, while the Transcription repressor GLI3R, which is generated in absence of smoothened, acts as a transcription repressor (PubMed:10375510). The repressor form (GLI3R) constitutes the major form of GLI3 (PubMed:10375510). |
| Cellular Location | Nucleus. Cytoplasm. Cell projection, cilium. Note=Full-length GLI3 is sequestered in the cytoplasm by SUFU in absence of smoothened signaling [Transcription activator GLI3]: Nucleus. Cytoplasm Cell projection, cilium Note=Sequestered in the cytoplasm by SUFU in absence of smoothened signaling (PubMed:38968120). Accumulates in primary cilia following smoothened activation: cilium localization requires the presence of KIF7 (PubMed:19592253, PubMed:20154143). It then translocates to the nucleus, where is activates transcription of target genes (PubMed:38968120). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441). TMEM216 reduces its nuclear localization (By similarity). {ECO:0000250|UniProtKB:Q61602, ECO:0000269|PubMed:11238441, ECO:0000269|PubMed:19592253, ECO:0000269|PubMed:20154143, ECO:0000269|PubMed:38968120} |
| Tissue Location | Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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