MCAK Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, FC, ICC
Primary Accession	Q99661
Reactivity	Human
Clonality	Monoclonal
Other Names	MCAK; KNSL6;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	81313 Da

Additional Information
Dilution	WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:100
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human MCAK
Description	Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	KIF2C
Synonyms	KNSL6
Function	In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end- on conversion of the chromosome-microtubule attachment (PubMed:23891108).
Cellular Location	Cytoplasm, cytoskeleton. Nucleus {ECO:0000250\|UniProtKB:P70096} Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. {ECO:0000250\|UniProtKB:P70096, ECO:0000269\|PubMed:17485487, ECO:0000269\|PubMed:21820309}
Tissue Location	Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis- specific.