MCM2 (1K1) Rabbit Monoclonal Antibody

Western blot detection of MCM2 in K562,C6,3T3,Hela cell lysates using MCM2 antibody(1:1000 diluted).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC
Primary Accession	P49736
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Calculated MW	101896 Da

Additional Information
Gene ID	4171
Other Names	DNA replication licensing factor MCM2, 3.6.4.12, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2 (HGNC:6944)
Dilution	WB~~1:1000 IHC~~1:100~500 IF~~1:50~200 FC~~1:10~50 ICC~~N/A
Storage Conditions	-20℃

Protein Information
Name	MCM2 (HGNC:6944)
Function	Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (PubMed:8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).
Cellular Location	Nucleus. Chromosome. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. {ECO:0000250\|UniProtKB:P55861}