Recombinant Anti-DSC3 Rabbit mAb

Western blot analysis of DSC3 expression in A431 (A) whole cell lysates. (Predicted band size: 100 kD; Observed band size: 100 kD)
Immunofluorescent analysis of DSC3 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	Q14574
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	99969 Da

Additional Information
Gene ID	1825
Other Names	CDHF3; DSC4; Desmocollin-3; Cadherin family member 3; Desmocollin-4; HT-CP
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human DSC3 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DSC3
Synonyms	CDHF3, DSC4
Function	A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (By similarity). Required for cell-cell adhesion in the epidermis, as a result required for the maintenance of the dermal cohesion and the dermal barrier function (PubMed:19717567). Required for cell-cell adhesion of epithelial cell layers surrounding the telogen hair club, as a result plays an important role in telogen hair shaft anchorage (By similarity). Essential for successful completion of embryo compaction and embryo development (By similarity).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome {ECO:0000250\|UniProtKB:P55850} Cytoplasm {ECO:0000250\|UniProtKB:P55850}. Note=Expressed in the cytoplasm and at the cell membrane of oocytes {ECO:0000250\|UniProtKB:P55850}
Tissue Location	Expressed throughout the basal and spinous layer of the epidermis with weak expression in the granular layer (at protein level) (PubMed:19717567, PubMed:7665906). Also expressed in the buccal mucosa, esophagus and cervix (at protein level) (PubMed:7665906)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.