Anti-Smooth Muscle Actin Antibody

Western blot analysis of Smooth Muscle Actin expression in EC (A), HUVE12 (B), A549 (C), NIH/3T3 (D), HL60 (E), Hela (F), K652 (G) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)
Immunohistochemical analysis of Smooth Muscle Actin staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-Smooth Muscle Actin Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P62736
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	42009 Da

Additional Information
Gene ID	59
Other Names	ACTSA; ACTVS; Actin, aortIC, smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Target/Specificity	Recombinant fusion protein of human Smooth Muscle Actin expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACTA2
Synonyms	ACTSA, ACTVS
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location	Cytoplasm, cytoskeleton.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.