Anti-CD157 Antibody

Western blot analysis of CD157 expression in rat spleen (A), mouse spleen (B) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 31 kD)
Immunohistochemical analysis of CD157 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HL60 cells using Anti-CD157 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	Q10588
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	35724 Da

Additional Information
Gene ID	683
Other Names	ADP-ribosyl cyclase/cyclIC, ADP-ribose hydrolase 2; ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; BST-1; CyclIC, ADP-ribose hydrolase 2; cADPr hydrolase 2; CD157
Target/Specificity	Recombinant fusion protein of human CD157 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BST1
Function	Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR) (PubMed:7805847). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).
Cellular Location	Cell membrane; Lipid-anchor, GPI-anchor
Tissue Location	Expressed in various tissues including placenta, lung, liver and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.