Anti-p16 INK4a Antibody

Western blot analysis of p16 INK4a expression in Hela (A), HepG2 (B), HEK293 (C) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 17 kD)
Immunohistochemical analysis of p16 INK4a staining in human endometrial cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-p16 INK4a Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P42771
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	16533 Da

Additional Information
Gene ID	1029
Other Names	CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A
Target/Specificity	Recombinant fusion protein of human p16 INK4a expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CDKN2A (HGNC:1787)
Synonyms	CDKN2, MTS1
Function	Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Cellular Location	Cytoplasm. Nucleus
Tissue Location	Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.