Anti-XRCC1 Antibody

Western blot analysis of XRCC1 expression in Hela (A), Jurkat (B), k562 (C), SKOV3 (D), COS7 (E) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 90 kD)
Immunohistochemical analysis of XRCC1 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of Jurkat cells using Anti-XRCC1 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P18887
Reactivity	Human, Mk
Host	Mouse
Clonality	Monoclonal
Calculated MW	69498 Da

Additional Information
Gene ID	7515
Other Names	DNA repair protein XRCC1; X-ray repair cross-complementing protein 1
Target/Specificity	Recombinant fusion protein of human XRCC1 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	XRCC1 {ECO:0000303\|PubMed:2247054, ECO:0000312\|HGNC:HGNC:12828}
Function	Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed:11163244, PubMed:28002403). Negatively regulates ADP- ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed:28002403, PubMed:34102106, PubMed:34811483). Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed:14500814, PubMed:34102106, PubMed:34811483).
Cellular Location	Nucleus. Chromosome Note=Moves from the nucleoli to the global nuclear chromatin upon DNA damage (PubMed:28002403). Recruited to DNA damage sites fowwing interaction with poly-ADP-ribose chains (PubMed:14500814)
Tissue Location	Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level)