癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
MGAT5 Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q09328 |
| Other Accession | Q09328 |
| Reactivity | Rat, Human, Mouse |
| Predicted | Rabbit, Chicken, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 84543 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human MGAT5 |
| Epitope Specificity | 201-300/741 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the glycosyltransferase 18 family. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | MGAT5 is a mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies. |
| Gene ID | 4249 |
|---|---|
| Other Names | GNT-V; GNT-VA; MGAT5A; glcNAc-T V; 4930471A21Rik; 5330407H02Rik; GlcNAc-TV; MGT5A_HUMAN; MGAT5; Alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase V; GlcNAc-T V (GNT-V); Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V; 2.4.1.155; GGNT5; MGT5A_MOUSE; Alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase; MGT5A_RAT; alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase; mannosyl (alpha-1, 6-)-glycoprotein beta-1, 6-N-acetyl-glucosaminyltransferase; alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | MGAT5 |
|---|---|
| Synonyms | GGNT5 |
| Function | Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides (PubMed:10395745, PubMed:30140003). Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2 (PubMed:10395745, PubMed:22614033, PubMed:30140003). Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N- glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity). Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis (PubMed:22614033). |
| Cellular Location | Golgi apparatus membrane {ECO:0000250|UniProtKB:P97259}; Single-pass type II membrane protein |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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