癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Afadin Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P55196 |
| Other Accession | P55196 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 206804 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Afadin |
| Epitope Specificity | 341-440/1824 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 dilute domain.Contains 1 FHA domain. Contains 1 PDZ (DHR) domain. Contains 2 Ras-associating domains |
| SUBUNIT | Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR. |
| DISEASE | Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12. |
| Gene ID | 4301 |
|---|---|
| Other Names | AF6; MLL-AF6; MLLT4; l-afadin; 5033403D15Rik; Af-6; Afadin; Gm314; I-afadin; S-afadin; AFAD_HUMAN; AFDN; ALL1-fused gene from chromosome 6 protein (Protein AF-6); Afadin adherens junction formation factor; AFAD_MOUSE; Protein Af-6; AFAD_RAT; afadin, adherens junction formation factor; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | AFDN (HGNC:7137) |
|---|---|
| Synonyms | AF6, MLLT4 |
| Function | Belongs to an adhesion system, probably together with the E- cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton (PubMed:11024295). May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions (PubMed:30463011). |
| Cellular Location | Cell junction, adherens junction. Note=Not found at cell-matrix AJs {ECO:0000250|UniProtKB:O35889} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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