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APPBP1 Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q13564 |
| Other Accession | Q13564 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 60246 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human APPBP1 |
| Epitope Specificity | 401-500/534 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. |
| SUBUNIT | Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2. |
| Post-translational modifications | Ubiquitinated by TRIP12, leading to its degradation by the proteasome. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | APPBP1 is a member of the ubiquitin-activating E1 family. In fetal tissues APPBP1 is widely expressed and in adult tissues it is expressed throughout the brain. APPBP1 is a cell membrane associated protein and functions as the regulatory subunit in a heterodimer with UBA3. The APPBP1/UBA3 complex binds to and activates NEDD8, a ubiquitin-like protein involved in signal transduction, cell proliferation and development. This suggests that APPBP1 affects a variety of cellular functions. In addition, APPBP1 is essential for cell cycle progression through the S/M checkpoint. More specifically, it inhibits the entry into S phase and promotes entry into M phase. |
| Gene ID | 8883 |
|---|---|
| Other Names | A-116A10.1; APPBP1; HPP1; NEDFIH; ula-1; 59kDa; ULA1_HUMAN; NAE1; Amyloid beta precursor protein-binding protein 1, 59 kDa (APP-BP1); Amyloid protein-binding protein 1; Proto-oncogene protein 1; ULA1_MOUSE; NEDD8 activating enzyme E1 subunit 1; amyloid beta precursor protein binding protein 1, 59kDa |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NAE1 |
|---|---|
| Synonyms | APPBP1 |
| Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development. |
| Cellular Location | Cell membrane. Note=Colocalizes with APP in lipid rafts |
| Tissue Location | Ubiquitous in fetal tissues. Expressed throughout the adult brain. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP98898
