癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Neutrophil Elastase Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q3UP87 |
| Other Accession | Q3UP87 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 28648 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Neutrophil Elastase |
| Epitope Specificity | 51-150/265 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. |
| SUBUNIT | Interacts with NOTCH2NL. |
| DISEASE | Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]. |
| Gene ID | 50701 |
|---|---|
| Other Names | ELA2; GE; HLE; HNE; NE; PMN-E; SCN1; F430011M15Rik; ELNE_HUMAN; ELANE; Bone marrow serine protease; Elastase-2; Human leukocyte elastase (HLE); Medullasin; PMN elastase; 3.4.21.37; ELNE_MOUSE; Leukocyte elastase; elastase, neutrophil expressed; elastase 2, neutrophil; neutrophil elastase; polymorphonuclear leukocyte elastase |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Elane |
|---|---|
| Synonyms | Ela2 |
| Function | Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes (PubMed:10947984). Shows microbicidal activity in neutrophils by mediating cleavage and degradation of bacterial proteins (By similarity). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). Involved for the formation of neutrophil extracellular traps (NETs): translocates into the nucleus downstream of MPO and mediates degadation of histone H4, thereby participating to chromatin decondensation (PubMed:20974816, PubMed:32045782). NETs are mainly composed of DNA fibers and are released by neutrophils to trap pathogens during inflammation (By similarity). Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (By similarity). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (By similarity). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (By similarity). |
| Cellular Location | Cytoplasmic vesicle, phagosome {ECO:0000250|UniProtKB:P08246}. Lysosome {ECO:0000250|UniProtKB:P08246}. Cytolytic granule {ECO:0000250|UniProtKB:P08246}. Nucleus {ECO:0000250|UniProtKB:P08246} Note=Localizes to azurophil granules, a type of primary lysosome found in neutrophils and monocytes that contains antimicrobial proteins (By similarity). Translocates from azurophil granules to the nucleus downstream of MPO during formation of neutrophil extracellular traps (NETs), and is present in released NETs (PubMed:32045782). Localized in phagolysosomes following ingestion of E.coli by neutrophils (By similarity). {ECO:0000250|UniProtKB:P08246, ECO:0000269|PubMed:32045782} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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