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ZWINT Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | O95229 |
| Other Accession | O95229 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 31293 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human ZWINT |
| Epitope Specificity | 85-170/277 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBUNIT | Interacts with ZW10 and MIS12. Interacts with the NDC80subunit of the NDC80 complex specifically during mitosis. Alsointeracts with CASC5, CETN3, DSN1 and PMF1. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | ZWINT is a component of the MIS12 complex, which is required for kinetochore function and spindle checkpoint activity. ZWINT interacts with ZW10 and targets it to the kinetochore at prometaphase. ZWINT is detectable on the kinetochore till late anaphase. Inhibition of ZWINT expression results in chromosome missegregation, spindle checkpoint failure and, ultimately, cell death upon cytokinesis. |
| Gene ID | 11130 |
|---|---|
| Other Names | ZWINT_HUMAN; ZWINT; ZW10 interactor; ZW10-interacting protein 1 (Zwint-1); HZwint-1; KNTC2AP; SIP30; ZWINT1; ZW10 interacting kinetochore protein; SNAP25 interacting protein of 30 kDa |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ZWINT |
|---|---|
| Function | Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:27881301, PubMed:38459127, PubMed:38459128). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:15094189, PubMed:15485811, PubMed:16732327). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:38459127, PubMed:38459128). Targets the RZZ complex to the kinetochore at prometaphase (PubMed:15485811). Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed:15485811). |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore Note=Localizes to kinetochores from late prophase to anaphase (PubMed:15502821, PubMed:27881301). Localizes to kinetochores both during mitosis and meiosis (By similarity) {ECO:0000250|UniProtKB:Q9CQU5, ECO:0000269|PubMed:15502821, ECO:0000269|PubMed:27881301} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP99220
