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IQGAP1 Recombinant Rabbit mAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P46940 |
| Other Accession | P46940 |
| Host | Rabbit |
| Clonality | Recombinant |
| Calculated MW | 189252 Da |
| Physical State | Liquid |
| Immunogen | A synthesized peptide derived from human IQGAP1 |
| Epitope Specificity | 1-58/1657 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| SIMILARITY | Contains 1 CH (calponin-homology) domain. Contains 4 IQ domains. Contains 1 Ras-GAP domain. Contains 1 WW domain. |
| SUBUNIT | Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. |
| Post-translational modifications | Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. |
| Gene ID | 8826 |
|---|---|
| Other Names | HUMORFA01; SAR1; p195; D7Ertd237e; D7Ertd257e; mKIAA0051; IQGA1_HUMAN; IQGAP1; KIAA0051; IQGA1_MOUSE; IQ motif containing GTPase activating protein 1; RasGAP-like with IQ motifs |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-200,IF=1:100-500,Flow-Cyt=1:50-100 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | IQGAP1 |
|---|---|
| Synonyms | KIAA0051 |
| Function | Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth (PubMed:15695813). May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed:20883816). |
| Cellular Location | Cell membrane. Nucleus. Cytoplasm. Cytoplasm, cell cortex {ECO:0000250|UniProtKB:Q9JKF1}. Apical cell membrane. Basolateral cell membrane {ECO:0000250|UniProtKB:Q9JKF1}. Note=Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816). |
| Tissue Location | Expressed in the placenta, lung, and kidney (PubMed:8051149). A lower level expression is seen in the heart, liver, skeletal muscle and pancreas (PubMed:8051149) |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP99553
