癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
WBSCR14 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9NP71 |
| Other Accession | Q9NP71 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 93073 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human WBSCR14/ChREBP |
| Epitope Specificity | 81-180/852 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 basic helix-loop-helix (bHLH) domain. |
| SUBUNIT | Binds DNA as a heterodimer with TCFL4/MLX. |
| Post-translational modifications | Phosphorylation at Ser-556 by AMPK inactivates the DNA-binding activity (By similarity). |
| DISEASE | Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | ChREBP (Carbohydrate responsive element binding protein) is a transcription factor playing a critical role in the nutrient and hormonal regulation of genes encoding enzymes of glucose metabolism and lipogenesis pathways. It contains several domains including a nuclear localization signal (NLS) near the N-terminus, polyproline domains, a basic helix-loop-helix leucine zipper (b/HLH/Zip) and a leucine zipper like (zip-like) domain. ChREBP is ubiquitously detected in various tissues, with highest expression in liver, kidney and white and brown adipose tissue. Under basal conditions ChREBP is localized in the cytosol, translocating into the nucleus upon high glucose stimulation following its dephosphorylation of phospho-serine 196. |
| Gene ID | 51085 |
|---|---|
| Other Names | CHREBP; MIO; MLX; MONDOB; WBSCR14; WS-bHLH; bHLHd14; MLXPL_HUMAN; MLXIPL; Class D basic helix-loop-helix protein 14 (bHLHd14); MLX interactor; MLX-interacting protein-like; WS basic-helix-loop-helix leucine zipper protein (WS-bHLH); Williams-Beuren syndrome chromosomal region 14 protein; MLX interacting protein like; Williams Beuren syndrome chromosome region 14; MLX interacting protein-like; carbohydrate response element binding protein |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | MLXIPL |
|---|---|
| Function | Glucose-responsive transcription activator that regulates fatty acid synthesis and glycolysis. Key determinant of systemic insulin sensitivity and glucose homeostasis. Important for the expression of fatty acid synthetic enzymes, including PC/Pcx, APOC4/Acl, ACACA/Acc1 and FASN/Fas (By similarity). Important for glucose-induced expression of L-type pyruvate kinase/PKLR (By similarity). Binds to the canonical and non-canonical E box DNA sequences 5'-CACGTG-3' and 5'-CACGCG-3' (By similarity). May also act as a transcriptional repressor (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q8VIP2}. Nucleus {ECO:0000250|UniProtKB:Q8VIP2}. Note=Localizes mainly in the cytoplasm of hepatocytes under low glucose and migrates into the nucleus under high glucose. {ECO:0000250|UniProtKB:Q8VIP2} |
| Tissue Location | Widely expressed with high levels in heart, brain, placenta, skeletal muscle and pancreas. Also expressed in fetal kidney, lung, liver and brain (PubMed:9860302). Expressed in fetal and adult liver (PubMed:10780788). Expressed in the cerebral cortex, including in the frontal, temporal, parietal and occipital lobes, and the cerebellum. Also detected in the intestine, including jejunum, ileum and colon (PubMed:11230181). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP99588
