癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
HIC5 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | O43294 |
| Other Accession | O43294 |
| Reactivity | Mouse |
| Predicted | Rat, Human, Rabbit, Chicken, Dog, Horse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 49814 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human TGFB1I1/HIC5 |
| Epitope Specificity | 31-130/461 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the paxillin family. Contains 4 LIM zinc-binding domains. |
| SUBUNIT | Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7 (By similarity). Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. |
| Post-translational modifications | Phosphorylated by gonadotropin-releasing hormone-activated SRC. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16. |
| Gene ID | 7041 |
|---|---|
| Other Names | ARA55; HIC-5; HIC5; TSC-5; TGFI1_BOVIN; TGFB1I1; Hydrogen peroxide-inducible clone 5 protein (Hic-5); TGFI1_HUMAN; Androgen receptor coactivator 55 kDa protein; Androgen receptor-associated protein of 55 kDa; Q9BPW3_HUMAN; Q3YBY7_MOUSE; Q3YBY8_MOUSE; Q3YBZ0_MOUSE; Q3YBZ1_MOUSE; Q3YBZ3_MOUSE; Q3YBZ4_MOUSE; Q3YBZ5_MOUSE; Q3YBZ6_MOUSE; TGFI1_MOUSE; TGF beta-stimulated clone 5 (TSC-5); TGFI1_RAT; transforming growth factor beta 1 induced transcript 1; hydrogen peroxide-inducible clone-5 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TGFB1I1 |
|---|---|
| Synonyms | ARA55 |
| Function | Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity. |
| Cellular Location | Cell junction, focal adhesion. Nucleus matrix. Cytoplasm, cytoskeleton. Note=Associated with the actin cytoskeleton; colocalizes with stress fibers |
| Tissue Location | Expressed in platelets, smooth muscle and prostate stromal cells (at protein level). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP99725
