癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
UCHL3 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P15374 |
| Other Accession | P15374 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 26183 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human UCHL3 |
| Epitope Specificity | 133-230/230 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the peptidase C12 family. |
| SUBUNIT | Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. |
| Post-translational modifications | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1 to 2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p14. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin. |
| Gene ID | 7347 |
|---|---|
| Other Names | UCH-L3; RGD1561196; UCHL3_HUMAN; UCHL3; Ubiquitin thioesterase L3; 3.4.19.12; UCHL3_MOUSE; UCHL3_RAT; ubiquitin C-terminal hydrolase L3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin thiolesterase |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | UCHL3 {ECO:0000303|PubMed:19047059, ECO:0000312|HGNC:HGNC:12515} |
|---|---|
| Function | Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins (PubMed:19154770, PubMed:21762696, PubMed:22689415, PubMed:2530630). Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8 (PubMed:19154770, PubMed:21762696, PubMed:22689415, PubMed:2530630, PubMed:9790970). Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains (PubMed:19154770, PubMed:21762696, PubMed:22689415, PubMed:2530630). Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurodegenerative disorders (By similarity). Involved in DNA repair via homologous recombination (HR) by mediating deubiquitination of RAD51 in response to DNA damage, promoting interaction between RAD51 and BRCA2 (PubMed:27941124). Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling (By similarity). Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis (By similarity). Required for stress-response retinal, skeletal muscle and germ cell maintenance (By similarity). May be involved in working memory (By similarity). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Highly expressed in heart, skeletal muscle, and testis. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP99791
