癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
KLHL2 Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | O95198 |
| Other Accession | O95198 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 65975 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human KLHL2 |
| Epitope Specificity | 1-100/593 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | KLHL2 is a 593 amino acid actin-binding protein. Highly expressed in brain, KLHL2 is thought to play a role in the organization of the actin cytoskeleton in brain cells. KLHL3 (kelch-like protein 3), is a 587 amino acid protein active in the protein ubiquitination pathway. KLHL3 is thought to be a substrate-specific adapter of an E3 ubiquitin-ligase complex that mediates the ubiquitination of target proteins, which leads to proteasomal degradation of the target protein. KLHL2 and KLHL3 proteins contain six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. |
| Gene ID | 11275 |
|---|---|
| Other Names | ABP-KELCH; MAV; MAYVEN; KLHL2_HUMAN; KLHL2; Actin-binding protein Mayven; kelch like family member 2; kelch (Drosophila)-like 2 (Mayven); kelch-like 2, Mayven (Drosophila) |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000,WB=1:500-2000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | KLHL2 {ECO:0000303|PubMed:23838290, ECO:0000312|HGNC:HGNC:6353} |
|---|---|
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, WNK1, WNK3 and WNK4, leading most often to their proteasomal degradation (PubMed:23838290). The BCR(KLHL2) complex catalyzes ubiquitination and degradation of NPTXR (By similarity). Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4 (PubMed:23838290). Plays a role in the reorganization of the actin cytoskeleton (PubMed:10397770). Promotes growth of cell projections in oligodendrocyte precursors (PubMed:15715669). |
| Cellular Location | Cytoplasm, cytoskeleton. Cell projection, ruffle {ECO:0000250|UniProtKB:Q8JZP3}. Cell projection. Cell projection, lamellipodium {ECO:0000250|UniProtKB:F1LZF0}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8JZP3}. Note=A proportion colocalizes with the actin cytoskeleton (PubMed:10397770). When over-expressed, colocalizes with NPTXR in perinuclear aggresomes (By similarity) {ECO:0000250|UniProtKB:Q8JZP3, ECO:0000269|PubMed:10397770} |
| Tissue Location | Ubiquitous. Detected throughout the brain. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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