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Anti-IL-5 Reference Antibody (mepolizumab)

Recombinant Antibody

Anti-IL-5 Reference Antibody (mepolizumab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-IL-5 Reference Antibody (mepolizumab) is 98.47%, determined by SEC-HPLC.
Immobilized human IL 5 His at 2 ug/mL can bind Anti-IL-5 Reference Antibody (mepolizumab), EC50=0.1188 ug/mL.
Anti-IL-5 Reference Antibody (mepolizumab) FACS Blocking was evaluated using Human IL-5Rβ-FL/HIL-5Rα CHOS. The IC50 was approximately 2.844 ug/mL.
Mepolizumab induced proliferation inhibition activity was evaluated using Human IL-5Rα TF-1 . The EC50 was approximately 0.3282 ug/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P05113
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	15238 Da

Additional Information
Target/Specificity	IL-5
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	IL5
Function	Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).
Cellular Location	Secreted.
Tissue Location	Present in peripheral blood mononuclear cells.