Anti-CSF2Rb / CD131 Reference Antibody (CSL311)

Recombinant Antibody

Anti-CSF2Rb / CD131 Reference Antibody (CSL311) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CSF2Rb / CD131 Reference Antibody (CSL311)is more than 98.34% ,determined by SEC-HPLC.
Immobilized human CSF2RB His at 2 µg/mL can bind Anti-CSF2Rb / CD131 Reference Antibody (CSL311)，EC50=0.0122 µg/mL
Human CSF2RB HEK293 cells were stained with Anti-CSF2Rb / CD131 Reference Antibody (CSL311) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC114=0.049 µg/mL
IL-3-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 0.2109 nM.
GM-CSF-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 4.105 nM.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P32927
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG4
Calculated MW	97336 Da

Additional Information
Target/Specificity	CSF2Rb / CD131
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CSF2RB
Synonyms	IL3RB, IL5RB
Function	Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA (PubMed:1495999). In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK- STAT pathway (PubMed:9516124).
Cellular Location	Membrane; Single-pass type I membrane protein.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.