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Anti-TNFRSF8 / CD30 Reference Antibody (brentuximab vedotin)

Recombinant Antibody

Anti-TNFRSF8 / CD30 Reference Antibody (brentuximab vedotin) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-TNFRSF8 / CD30 Reference Antibody (brentuximab vedotin) is 98.99%, determined by SEC-HPLC.
Immobilized human CD30 His at 2 ug/mL can bind Anti-TNFRSF8 / CD30 Reference Antibody (brentuximab vedotin), EC50 is 0.006007 ug/mL.
MMAE is conjugated by sulfhydryl coupling,the DAR value of Anti-TNFRSF8 / CD30 Reference Antibody (brentuximab vedotin) antibody is 4.0.
Human TNFRSF8 / CD30 EGFP CHO Cell Line were stained with Anti-TNFRSF8 / CD30 Reference Antibody (brentuximab vedotin) and negative control protein respectively, washed and then followed by PE and analyzed with FACS,The EC50 is 0.12 µg/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P28908
Reactivity	Human
Clonality	ADC
Isotype	IgG1
Calculated MW	63747 Da

Additional Information
Target/Specificity	TNFRSF8 / CD30
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	MMAE
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	TNFRSF8 (HGNC:11923)
Function	Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa- B (PubMed:8999898).
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein
Tissue Location	[Isoform 2]: Detected in alveolar macrophages (at protein level).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol