Anti-OSMR Reference Antibody (vixarelimab)

Recombinant Antibody

Anti-OSMR Reference Antibody (vixarelimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-OSMR Reference Antibody (vixarelimab)is more than 96.9% ,determined by SEC-HPLC.
Immobilized human OSMR His at 2 µg/mL can bind Anti-OSMR Reference Antibody (vixarelimab)，EC50=0.0024 µg/mL
Human OSMR HEK293 cells were stained with Anti-OSMR Reference Antibody (vixarelimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC261=0.0359 µg/mL
Anti-OSMR Reference Antibody (vixarelimab) P-STAT3 Assay was evaluated using NHDF. The IC50 was approximately 0.3493 nM.
Anti-OSMR Reference Antibody (vixarelimab) MCP-1 secretion was evaluated using NHDF. The IC50 was approximately 0.0812 nM.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q99650
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG4SP
Calculated MW	110509 Da

Additional Information
Target/Specificity	OSMR
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	OSMR
Synonyms	OSMRB
Function	Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events.
Cellular Location	Membrane; Single-pass type I membrane protein
Tissue Location	Expressed in keratinocytes (at protein level) (PubMed:21261663). Expressed at relatively high levels in all neural cells as well as fibroblast and epithelial cells (PubMed:8999038)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.