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Anti-PSGL1 / CD162 Reference Antibody (neihulizumab)

Recombinant Antibody

Anti-PSGL1 / CD162 Reference Antibody (neihulizumab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-PSGL1 / CD162 Reference Antibody (neihulizumab) is 99.48%, determined by SEC-HPLC.
Human PSGL1 / CD162 HEK293T Cell Line were stained with Anti-PSGL1 / CD162 Reference Antibody (neihulizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q14242
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG4SP
Calculated MW	43201 Da

Additional Information
Target/Specificity	PSGL1 / CD162
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	SELPLG
Function	A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Expressed on neutrophils, monocytes and most lymphocytes

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol