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Anti-ROBO1 Reference Antibody (Asclepius Technology patent anti-Robo1 CAR)

Recombinant Antibody

Anti-ROBO1 Reference Antibody (Asclepius Technology patent anti-Robo1 CAR) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-ROBO1 Reference Antibody (Asclepius Technology patent anti-Robo1 CAR) is more than 95%, determined by SEC-HPLC.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q9Y6N7
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	180930 Da

Additional Information
Target/Specificity	ROBO1
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	ROBO1
Synonyms	DUTT1
Function	Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell projection, axon {ECO:0000250\|UniProtKB:O89026}. Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000250\|UniProtKB:O55005}; Single-pass membrane protein {ECO:0000250\|UniProtKB:O55005} Note=Detected at growth cones in thalamus neurons. Detected at growth cones in thalamus neurons (By similarity). PRRG4 prevents cell surface location and both colocalize in the Endoplasmic reticulum/Golgi adjacent to the cell nucleus (By similarity) {ECO:0000250\|UniProtKB:O55005, ECO:0000250\|UniProtKB:O89026}
Tissue Location	Widely expressed, with exception of kidney.