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Anti-CLDN1 Reference Antibody (Eclutatug)

Anti-CLDN1 Reference Antibody (Eclutatug) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-CLDN1 Reference Antibody (Eclutatug) is 99.84%, determined by SEC-HPLC.
Immobilized human-CLDN1-VLP at 2 µg/mL can bind Eclutatug, EC50= 0.03373 µg/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	O95832
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1-REM-noK
Calculated MW	22744 Da

Additional Information
Target/Specificity	CLDN1 / CLD1 / SEMP1
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	CLDN1
Synonyms	CLD1, SEMP1
Function	Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
Cellular Location	Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane Note=Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.
Tissue Location	Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol