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Anti-PAR1 Reference Antibody (Novartis patent anti-PAR1)

Anti-PAR1 Reference Antibody (Novartis patent anti-PAR1) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-PAR1 Reference Antibody (Novartis patent anti-PAR1) is 100%, determined by SEC-HPLC.
PLVX-puro-hu CCR1-E-coil cell line were stained with Novartis patent anti-PAR1 and negative control protein respectively, washed and then followed by PE and analyzed with FACS, with the EC50 is 0.631 nM.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P25116
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	47441 Da

Additional Information
Target/Specificity	PAR1
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	F2R (HGNC:3537)
Synonyms	CF2R, PAR1, TR
Function	High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed:1672265, PubMed:8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5- trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L- glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed:10079109). Mediates up- regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed:30568593, PubMed:34831181).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P26824}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P26824}
Tissue Location	Platelets and vascular endothelial cells.