癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
FLASH Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q9UKL3 |
| Other Accession | AF165161, 5533376 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 222658 Da |
| Conjugate | Unconjugated |
| Application Notes | FLASH antibody can be used for detection of FLASH by Western blot at 0.5 µg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 10 µg/mL. |
| Gene ID | 9994 |
|---|---|
| Other Names | FLASH Antibody: CED-4, FLASH, RIP25, KIAA1315, CASP8-associated protein 2, FLICE-associated huge protein, caspase 8 associated protein 2 |
| Target/Specificity | CASP8AP2; |
| Reconstitution & Storage | FLASH antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | FLASH Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | CASP8AP2 (HGNC:1510) |
|---|---|
| Function | Participates in TNF-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B (PubMed:12477726, PubMed:15698540). Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation (PubMed:12477726, PubMed:15698540). Involved in TNF-induced activation of NF-kappa-B via a TRAF2-dependent pathway (By similarity). Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B (PubMed:17245429). Required for the activation of CASP8 in FAS-mediated apoptosis (PubMed:17245429). Involved for histone gene transcription and progression through S phase: required for the generation of mature histone mRNAs following the 3' end cleavage of histone pre-mRNAs (PubMed:17003125, PubMed:19854135, PubMed:32367068). |
| Cellular Location | Cytoplasm. Nucleus. Nucleus, PML body. Mitochondrion. Note=Exported from the nucleus to the mitochondria upon FAS activation |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
FLASH Antibody: A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.
REFERENCES
Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85
Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3
Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800)
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