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DAPK2 Antibody

     
  • 1 - DAPK2 Antibody ASC10117
    Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 µg/mL.
  • 2 - DAPK2 Antibody ASC10117
    Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 µg/mL.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E, IHC-P
Primary Accession Q9UIK4
Other Accession BAA88063, 6521210
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 42898 Da
Conjugate Unconjugated
Application Notes DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 µg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 µg/mL.
Additional Information
Gene ID 23604
Other Names DAPK2 Antibody: DRP1, DRP-1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase 2, death-associated protein kinase 2
Target/Specificity DAPK2; DAPK2 has no cross responses to DAPK1.
Reconstitution & Storage DAPK2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsDAPK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAPK2
Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).
Cellular Location Cytoplasm. Cytoplasmic vesicle, autophagosome lumen
Tissue Location Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

DAPK2 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.

REFERENCES

Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80
Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)

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