癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SCRAPPER Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q96IG2 |
| Other Accession | NP_116264, 27734755 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 48423 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 84961 |
|---|---|
| Other Names | F-box/LRR-repeat protein 20, F-box and leucine-rich repeat protein 20, F-box/LRR-repeat protein 2-like, FBXL20, FBL2 |
| Target/Specificity | FBXL20; |
| Reconstitution & Storage | SCRAPPER antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SCRAPPER Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FBXL20 |
|---|---|
| Synonyms | FBL2 |
| Function | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Role in neural transmission (By similarity). |
| Cellular Location | Cytoplasm. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
REFERENCES
Ho MS, Tsai PI, and Chien CT. F-box proteins: the key to protein degradation. J. Biomed. Sci.2006; 13:181-91.
Dobie F and Craig AM. A fight for neurotransmission: SCRAPPER trashes RIM. Cell2007; 130:775-7.
Yao I, Takagi H, Ageta H, et al. SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release. Cell2007; 130:943-57.
Ding M, Chao D, Wang G, et al. Spatial regulation of an E3 ubiquitin ligase directs selective synapse elimination. Science2007; 317:947-51.
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