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CCDC47 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q96A33 |
| Other Accession | EAW94279, 119614685 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 55874 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | CCDC47 antibody can be used for detection of CCDC47 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 57003 |
|---|---|
| Other Names | Coiled-coil domain-containing protein 47, CCDC47 |
| Target/Specificity | CCDC47; |
| Reconstitution & Storage | CCDC47 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | CCDC47 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CCDC47 {ECO:0000303|PubMed:30401460, ECO:0000312|HGNC:HGNC:24856} |
|---|---|
| Function | Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32814900, PubMed:32820719, PubMed:36261522). The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed:36261522). Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein (By similarity). Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex (By similarity). Involved in the regulation of calcium ion homeostasis in the ER (PubMed:30401460). Required for proper protein degradation via the ERAD (ER-associated degradation) pathway (PubMed:25009997). Has an essential role in the maintenance of ER organization during embryogenesis (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Rough endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9D024}; Single-pass type I membrane protein |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CCDC47 Antibody: The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. The CCDC47 gene maps to chromosome 17 at 17q23.3. Little is known about this single-pass membrane protein except that the coiled-coil domain is within the cytosolic domain near the carboxy terminus.
REFERENCES
Steinmetz MO, Jelesarov I, Matousek WM, et al. Molecular basis of coiled-coil formation. Proc. Natl. Acad. Sci. USA2007; 104:7062-7.
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