癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ZEB2 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | O60315 |
| Other Accession | AAI27103, 117558786 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 136447 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 9839 |
|---|---|
| Other Names | Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, SMADIP1, Zinc finger homeobox protein 1b, ZEB2, KIAA0569, SIP1, ZFHX1B, ZFX1B |
| Target/Specificity | ZEB2; ZEB2 antibody is predicted to not cross-react with ZEB1. |
| Reconstitution & Storage | ZEB2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ZEB2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ZEB2 (HGNC:14881) |
|---|---|
| Function | Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters (PubMed:16061479, PubMed:20516212). Represses transcription of E-cadherin (PubMed:16061479). Represses expression of MEOX2 (PubMed:20516212). |
| Cellular Location | Nucleus. Chromosome |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ZEB2 Antibody: ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.
REFERENCES
Comjin J, Berx G, Vermassen P, et al. The two-handed E box binding zinc finger protein SIP1 down regulates E-cadherin and induces invasion. Mol. Cell2001; 7:1267-78.
Sayan AE, Griffiths TR, Pal R, et al. SIP1 protein protects cells from DNA damage-induced apoptosis and has independent prognostic value in bladder cancer. Proc. Natl. Acad. Sci. USA2009; 106:14884-9.
Oztas E, Avci ME, Ozcan A, et al. Novel monoclonal antibodies detect Smad-interacting protein 1 (SIP1) in the cytoplasm of human cells from multiple tumor tissue arrays. Exp. Mol. Pathol.2010; epub
Wakamatsu N, Yamada Y, Yamada K, et al. Mutations in SIP1, encoding Smad interacting protein-1, cause a form of Hirschsprung disease. Nat. Genet.2001; 27:369-70.
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